Microbiology 100 Interview Questions

1. Why does MCB incubate at 42–44 degrees Celsius?

2. Why is a 0.45-micron filter used for water analysis? why not 0.2 microns?

3. Why is R2A used for water analysis?

4. What is Data integrity?

5. Explain about ALCOA+?

6. Why is Salmonella detection only 10 gram sample for MLT Why not 1 gram?

7. Which instrument is used in the microbiology department, and what is its make?

8. How are you performing autoclave calibration?

9. Explain about autoclave revalidation.

10. Which method you are using for water analysis?

11. Explain about your water sampling?

12. What are the limits of environmental monitoring?

13. What are the limits of purified water and treated water?

14. What is the Water USP chapter?
 
15. What is the Environmental Monitoring USP chapter?

16. Explain about water system qualification?

17. What is the Compressed gas USP chapter?

18. How are you doing GPT ( Growth promotion test)?

19. Explain about MLT?

20. What are the responsibilities and roles in your lab?

21. What is the F0 value?

22. What are alert and action limits?

23. How to set alert and action limits and formulas?

24. What are trend charts?

25. Explain differential pressure in the pharmaceutical industry.

26. Explain about incubator temperature and limits.

27. Can you explain the pathogen test in MLT?

28. How you are selecting an environmental monitoring location?

29. How you are doing nitrogen gas monitoring?

30. What would you do next if colonies were observed in compressed air sampling?

31. Can you explain gram staining and its principles?

32. Tell me about 5 gram-positive and gram-negative bacteria.

33. Why RVSEB sterilization at 115 degree temperature?

34. Have you seen the Vitek report and what details are included in it?

35. Why do EM plates incubate first at 20-25 degrees and then at 30-35 degree temperature?

36. Why do R2A plates incubate at 30- 35 degree temperature for 5 days?

37. How do you decontaminate the media?

38. How do you fumigation microbiology lab?

39. After fumigation you exposing the EM plates?

40. Which solution is used for fumigation?

41. What is the composition of minncare,dettol?

42. Why are plates incubated inverted position?

43. Why are you using saline for swabs?

44. How much measurement swab are you taking for the instrument?

45. Why water sample storage in 2-8 degree temperature?

46. Explain about monthly calibration of balance and limits.

47. Explain about oxidation test.

48. How do you perform pathogen confirmation tests?

49. How are you selecting an EM location in the manufacturing area?

50. Explain about revalidation of LAF?

51. What is the difference between a laminar air flow and a biosafety cabinet?

52. What is the temperature inside the microbiology lab?

53. How are you receiving new media in the microbiology lab?

54. How many passages do you use in microbiology?

55. Why 5 passages only?

56. How many sensors are installed inside the autoclave for revalidation?

57. How many hours of burning a UV lamp after your change in LAF?

58. Why 70% IPA you are using why not 100%?

59. How are you calibrating the micropipettes?

60. How much media weighing for SCDA and it's PH?

61. Why you are not using SCDA for water testing?

62. What is the limit of TOC?

63. Did you see the TOC instrument at the water plant, and what is the limit inside the display?

64. How much time do you flush the water for sampling?

65. How you are doing TOC sampling?

66. Why are you exposing EM plates for 4 hours?

67. Why you are doing air sampling for 10 minutes for 1000 litres of air which the USP chapter mentioned?

68. Water system validation where it is mentioned 2-4 Weeks for phase 1?

69. Why autoclave 121-degree sterilization for 26 minutes or 15 minutes?

70. Explain the growth promotion test for SCDA.

71. What exactly are you checking every day to verify PLC reports?

72. What is validation?

73. How do you perform new instrument qualification?

74. How many incubators are there in your lab?

75. Due done any mapping for incubators?

76. How to release EM plates?

77. Why in MLT 1:100 dilution you are making?

78. Why are 10 grams only you are using for MLT?

79. What are the limits of MLT raw materials and finished products?

80. What is BET?

81. What is sterility?

82. What is media filling?

83. If autoclave breakdown what will you do next step?

84. How do you ensure autoclave every cycle is properly sterilized or not?

85. If environmental monitoring crosses the alert limit what will you do?

86. How do you report the excursion in the microbiology department?

87. How do you conclude there is no laboratory error in microbiology? how is your investigation micro analyst?

88. How do you perform E coli test?

89. Explain about RODAC?

90. What is the size of the petri plates you are using in the microbiology lab?

91. what is the temperature of the hot air oven for drying glassware?

92. Explain about the coliform test.

93. What is the filler correction and how many holes are there in the air sampler sieve?

94. Explain about deviation.

95. What Water doll test you are doing in EM?

96. Explain how to culture storage and handling.

97. Why was treated water sampling before they added in bottle sodium thiosulphate and sterilization?

98. What is the hold period of media?

99. Why does the media cool to 45 degrees before pouring the plates?

100. What is 21 CFR part 11?

 

Visit our Service: https://likeways.co.in/resume-build-and-review/